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Adaptive laboratory evolution of microbial co-cultures for improved metabolite secretion.

ABSTRACT: Adaptive laboratory evolution has proven highly effective for obtaining microorganisms with enhanced capabilities. Yet, this method is inherently restricted to the traits that are positively linked to cell fitness, such as nutrient utilization. Here, we introduce coevolution of obligatory mutualistic communities for improving secretion of fitness-costly metabolites through natural selection. In this strategy, metabolic cross-feeding connects secretion of the target metabolite, despite its cost to the secretor, to the survival and proliferation of the entire community. We thus co-evolved wild-type lactic acid bacteria and engineered auxotrophic Saccharomyces cerevisiae in a synthetic growth medium leading to bacterial isolates with enhanced secretion of two B-group vitamins, viz., riboflavin and folate. The increased production was specific to the targeted vitamin, and evident also in milk, a more complex nutrient environment that naturally contains vitamins. Genomic, proteomic and metabolomic analyses of the evolved lactic acid bacteria, in combination with flux balance analysis, showed altered metabolic regulation towards increased supply of the vitamin precursors. Together, our findings demonstrate how microbial metabolism adapts to mutualistic lifestyle through enhanced metabolite exchange.

SUBMITTER: Konstantinidis D

PROVIDER: S-EPMC8351387 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Adaptive Laboratory Evolution of microbial co-cultures for improved metabolite secretion

Project description:Small microbial mutualistic communities consisting of lactic acid bateria and S. cerevisiae evolved together for increased vitamin secretion from the lactic acid bacteria. Proteomics analysis of the parental and evolved bacteria to identify differences that occured during the prolonged co-evolution.

2021-08-05 | PXD022250 | Pride

Adaptive laboratory evolution of microbial co-cultures for improved metabolite secretion

Project description:<p>Adaptive laboratory evolution has proven highly effective for obtaining microorganisms with enhanced capabilities. Yet, this method is inherently restricted to the traits that are positively linked to cell fitness, such as nutrient utilization. Here, we introduce coevolution of obligatory mutualistic communities for improving secretion of fitness-costly metabolites through natural selection. In this strategy, metabolic cross-feeding connects secretion of the target metabolite, despite its cost to the secretor, to the survival and proliferation of the entire community. We thus co-evolved wild-type lactic acid bacteria and engineered auxotrophic <em>Saccharomyces cerevisiae</em> in a synthetic growth medium leading to bacterial isolates with enhanced secretion of two B-group vitamins, viz., riboflavin and folate. The increased production was specific to the targeted vitamin, and evident also in milk, a more complex nutrient environment that naturally contains vitamins. Genomic, proteomic and metabolomic analyses of the evolved lactic acid bacteria, in combination with flux balance analysis, showed altered metabolic regulation towards increased supply of the vitamin precursors. Together, our findings demonstrate how microbial metabolism adapts to mutualistic lifestyle through enhanced metabolite exchange.</p>

2021-07-02 | MTBLS2217 | MetaboLights

Adaptive laboratory evolution with heterologous pgi

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Adaptive laboratory evolution of chorismate synthase knockouts

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Adaptive Laboratory Evolution of Cupriavidus necator H16 for Carbon Co-Utilization with Glycerol.

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| S-EPMC6888959 | biostudies-literature

Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution.

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| S-EPMC6599523 | biostudies-literature

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