Unknown

Dataset Information

0

T cell stiffness is enhanced upon formation of immunological synapse.


ABSTRACT: T cells are activated by target cells via an intimate contact, termed immunological synapse (IS). Cellular mechanical properties, especially stiffness, are essential to regulate cell functions. However, T cell stiffness at a subcellular level at the IS still remains largely elusive. In this work, we established an atomic force microscopy (AFM)-based elasticity mapping method on whole T cells to obtain an overview of the stiffness with a resolution of ~60 nm. Using primary human CD4+ T cells, we show that when T cells form IS with stimulating antibody-coated surfaces, the lamellipodia are stiffer than the cell body. Upon IS formation, T cell stiffness is enhanced both at the lamellipodia and on the cell body. Chelation of intracellular Ca2+ abolishes IS-induced stiffening at the lamellipodia but has no influence on cell-body-stiffening, suggesting different regulatory mechanisms of IS-induced stiffening at the lamellipodia and the cell body.

SUBMITTER: Jung P 

PROVIDER: S-EPMC8360652 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3284572 | biostudies-literature
| S-EPMC2772177 | biostudies-literature
| S-EPMC1458636 | biostudies-literature
| S-EPMC357034 | biostudies-literature
| S-EPMC3823004 | biostudies-literature
| S-EPMC4258405 | biostudies-literature
| S-EPMC137538 | biostudies-literature
| S-EPMC3131438 | biostudies-literature
| S-EPMC3275606 | biostudies-literature
| S-EPMC7874230 | biostudies-literature