Nanopore sequencing reveals endogenous NMD-targeted isoforms in human cells.

ABSTRACT:

Background

Nonsense-mediated mRNA decay (NMD) is a eukaryotic, translation-dependent degradation pathway that targets mRNAs with premature termination codons and also regulates the expression of some mRNAs that encode full-length proteins. Although many genes express NMD-sensitive transcripts, identifying them based on short-read sequencing data remains a challenge.

Results

To identify and analyze endogenous targets of NMD, we apply cDNA Nanopore sequencing and short-read sequencing to human cells with varying expression levels of NMD factors. Our approach detects full-length NMD substrates that are highly unstable and increase in levels or even only appear when NMD is inhibited. Among the many new NMD-targeted isoforms that our analysis identifies, most derive from alternat

SUBMITTER: Karousis ED 

PROVIDER: S-EPMC8361881 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature