Spinal astrocyte aldehyde dehydrogenase-2 mediates ethanol metabolism and analgesia in mice.

ABSTRACT:

Background

Little is known about the targets in the CNS that mediate ethanol analgesia. This study explores the role of spinal astrocyte aldehyde dehydrogenase-2 (ALDH2), a key ethanol-metabolising enzyme, in the analgesic effects of ethanol in mice.

Methods

Astrocyte and hepatocyte ALHD2-deficient mice were generated and tested in acute and chronic pain models. Cell-type-specific distribution of ALDH2 was analysed by RNA in situ hybridisation in spinal slices from astrocytic ALDH2-deficient mice and their wild-type littermates. Spinal ethanol metabolites and γ-aminobutyric acid (GABA) content were measured using gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry.

Results

ALDH2 mRNA was expressed in both astrocytes and neurones in spinal

SUBMITTER: Jin S 

PROVIDER: S-EPMC8362281 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature