Ontology highlight
ABSTRACT: Background
The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations.Methods
Here we present a cost-efficient and fast workflow combining a prescreening of SARS-CoV-2-positive samples using reverse transcription polymerase chain reaction melting curve analysis with multiplexed IP-RP-HPLC-based single nucleotide primer extensions.Results
The entire workflow from positive SARS-CoV-2 testing to base-specific identification of variants requires about 24 hours.Conclusions
We applied the sensitive method to monitor local variant of concern outbreaks in SARS-CoV-2-positive samples collected in a confined region of Germany.
SUBMITTER: Tierling S
PROVIDER: S-EPMC8364759 | biostudies-literature | 2021 Aug
REPOSITORIES: biostudies-literature
Tierling Sascha S Kattler Kathrin K Vogelgesang Markus M Pfuhl Thorsten T Lohse Stefan S Lo Porto Christina C Schmitt Beate B Nastasja Seiwert S Salhab Abdulrahman A Smola Sigrun S Walter Jörn J
Open forum infectious diseases 20210714 8
<h4>Background</h4>The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations.<h4>Methods</h4>Here we present a cost-efficient and fast workflow combining a prescreening of SARS-CoV-2-positive samples using reverse transcription polymerase chain reaction melting curve analysis with multiplexed IP-RP-HPLC-based single nucleotide primer extensions.<h4>Results</h4>The entire wor ...[more]