Interleukin 32 Promotes Foxp3⁺ Treg Cell Development and CD8⁺ T Cell Function in Human Esophageal Squamous Cell Carcinoma Microenvironment.

ABSTRACT: Proinflammatory cytokine interleukin 32 (IL-32) is involved in infectious diseases and cancer, but what subtypes of immune cells express IL-32 and its roles in tumor microenvironment (TME) have not been well discussed. In this study, we applied bioinformatics to analyze single-cell RNA sequencing data about tumor-infiltrating immune cells from esophageal squamous cell carcinoma (ESCC) TME and analyzed IL-32 expression in different immune cell types. We found CD4⁺ regulatory T cells (Treg cells) express the highest level of IL-32, while proliferating T and natural killer cells expressed relatively lower levels. Knocking down of IL-32 reduced Foxp3 and interferon gamma (IFNγ) expressions in CD4⁺ and CD8⁺ T cells, respectively. IL-32 was positively correlated

SUBMITTER: Han L 

PROVIDER: S-EPMC8369465 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature