Ontology highlight
ABSTRACT:
SUBMITTER: Emperle M
PROVIDER: S-EPMC8373138 | biostudies-literature | 2021 Aug
REPOSITORIES: biostudies-literature
Emperle Max M Bangalore Disha M DM Adam Sabrina S Kunert Stefan S Heil Hannah S HS Heinze Katrin G KG Bashtrykov Pavel P Tessmer Ingrid I Jeltsch Albert A
Nucleic acids research 20210801 14
DNMT3A/3L heterotetramers contain two active centers binding CpG sites at 12 bp distance, however their interaction with DNA not containing this feature is unclear. Using randomized substrates, we observed preferential co-methylation of CpG sites with 6, 9 and 12 bp spacing by DNMT3A and DNMT3A/3L. Co-methylation was favored by AT bases between the 12 bp spaced CpG sites consistent with their increased bending flexibility. SFM analyses of DNMT3A/3L complexes bound to CpG sites with 12 bp spacing ...[more]