Live long and active: Polypeptide-mediated assembly of antibody variable fragments.

ABSTRACT: Antibodies possess multiple biologically relevant features that have been engineered into new therapeutic formats. Two examples include the adaptable specificity of their variable (Fv) region and the extension of plasma circulation times through their crystallizable (Fc) region. Since the invention of the single chain variable fragment (scFv) in 1988, antibody variable regions have been re-engineered into a wide variety of multifunctional nanostructures. Among these strategies, peptide-mediated self-assembly of variable regions through heterologous expression has become a powerful method to produce homogenous, functional biomaterials. This manuscript reviews recent reports of antibody fragments assembled through fusion with peptides and proteins, including elastin-like polypeptides (ELPs),

SUBMITTER: Lee C 

PROVIDER: S-EPMC8382037 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature