Unknown

Dataset Information

0

TCA Cycle and Its Relationship with Clavulanic Acid Production: A Further Interpretation by Using a Reduced Genome-Scale Metabolic Model of Streptomyces clavuligerus.


ABSTRACT: Streptomyces clavuligerus (S. clavuligerus) has been widely studied for its ability to produce clavulanic acid (CA), a potent inhibitor of β-lactamase enzymes. In this study, S. clavuligerus cultivated in 2D rocking bioreactor in fed-batch operation produced CA at comparable rates to those observed in stirred tank bioreactors. A reduced model of S. clavuligerus metabolism was constructed by using a bottom-up approach and validated using experimental data. The reduced model was implemented for in silico studies of the metabolic scenarios arisen during the cultivations. Constraint-based analysis confirmed the interrelations between succinate, oxaloacetate, malate, pyruvate, and acetate accumulations at high CA synthesis rates in submerged cultures of S. clavuligerus. Further analysis using shadow prices provided a first view of the metabolites positive and negatively associated with the scenarios of low and high CA production.

SUBMITTER: Ramirez-Malule H 

PROVIDER: S-EPMC8389198 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC10654394 | biostudies-literature
| S-EPMC310172 | biostudies-literature
| S-EPMC5625738 | biostudies-literature
| S-EPMC5034143 | biostudies-literature
| S-EPMC7569809 | biostudies-literature
| S-EPMC3818869 | biostudies-literature
| S-EPMC3958168 | biostudies-literature
2018-08-30 | GSE119208 | GEO
| S-EPMC6382702 | biostudies-literature
| S-EPMC2593231 | biostudies-literature