DNA-facilitated target search by nucleoproteins: Extension of a biosensor-surface plasmon resonance method.

ABSTRACT: To extend the value of biosensor-SPR in the characterization of DNA recognition by nucleoproteins, we report a comparative analysis of DNA-facilitated target search by two ETS-family transcription factors: Elk1 and ETV6. ETS domains represent an attractive system for developing biosensor-based techniques due to a broad range of physicochemical properties encoded within a highly conserved DNA-binding motif. Building on a biosensor approach in which the protein is quantitatively sequestered and presented to immobilized cognate DNA as nonspecific complexes, we assessed the impact of intrinsic cognate and nonspecific affinities on long-range (intersegmental) target search. The equilibrium constants of DNA-facilitated binding were sensitive to the intrinsic binding properties of the proteins su

SUBMITTER: Vo TD 

PROVIDER: S-EPMC8427768 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature