Browse
Submit Data
Databases
API
Help

Dataset Information

9 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs.

ABSTRACT: PIWI-interacting RNAs (piRNAs) support the germline by suppressing retrotransposons. Studies of the pathway in mice have strongly shaped the view that mammalian piRNAs are essential for male but not for female fertility. Here, we report that the role of the piRNA pathway substantially differs in golden hamsters (Mesocricetus auratus), the piRNA pathway setup of which more closely resembles that of other mammals, including humans. The loss of the Mov10l1 RNA helicase-an essential piRNA biogenesis factor-leads to striking phenotypes in both sexes. In contrast to mice, female Mov10l1^-/- hamsters are sterile because their oocytes do not sustain zygotic development. Furthermore, Mov10l1^-/- male hamsters have impaired establishment of spermatogonia accompanied by transcriptome dysregulation and an expression surge of a young retrotransposon subfamily. Our results show that the mammalian piRNA pathway has essential roles in both sexes and its adaptive nature allows it to manage emerging genomic threats and acquire new critical roles in the germline.

SUBMITTER: Loubalova Z

PROVIDER: S-EPMC8437802 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters

Project description:Many animals have a conserved adaptive genome defense system known as the Piwi-interacting RNA (piRNA) pathway which is essential for germ cell development and function. Disruption of individual mouse Piwi genes results in male but not female sterility, leading to the assumption that PIWI genes do not play a role in mammalian oocytes. Contrary to this, PIWIL1- and PIWIL3-defective golden hamsters we have produced show defects in production of functional oocytes. Mechanisms involved, however, vary between the two PIWI genes; lack of PIWIL1 has a strong impact on gene expression including hamster-specific young transposon de-silencing, while PIWIL3 deficiency has little impact on gene expression in oocytes, although DNA methylation of chromosomes was reduced to some extent. Our findings serve as the foundation for developing useful models to study the piRNA pathway in mammalian oocytes including humans, which cannot be possible with mice.

2021-07-02 | GSE164356 | GEO

Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters

Project description:Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters

| PRJNA690097 | ENA

Golden hamster piRNAs are essential for early development and establishment of spermatogonia.

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-07-13 | GSE164658 | GEO

Golden hamster piRNAs are essential for early development and establishment of spermatogonia [hamster_oocyte.small_RNAseq]

Project description:PIWI-associated RNAs (piRNAs) support germline by suppressing retrotransposons and genes. In mice, piRNAs are essential for spermatogenesis but not oogenesis. To test how this applies to other mammals, we deleted Mov10l1 helicase in golden hamsters whose piRNA pathway configuration is closer to other mammals than that of mice. Mov10l1–/– male hamsters showed impaired establishment of spermatogonia accompanied by transcriptome dysregulation and surge of MYSERV retrotransposon expression. The rare viable spermatogenic cells showed meiotic failure phenotype like Mov10l1–/– mice. Female Mov10l1–/–hamsters were sterile due to post-meiotic loss of developmental competence in zygotes. Unique phenotypes of Mov10l1–/– hamsters demonstrate adaptive nature of piRNA-mediated control of genes and retrotransposons, which enables confronting emerging genomic threats or acquiring new physiological roles.

2021-07-13 | GSE174819 | GEO

Golden hamster piRNAs are essential for early development and establishment of spermatogonia [hamster_oocyte.bisulfite_seq]

2021-07-13 | GSE164657 | GEO

Golden hamster piRNAs are essential for early development and establishment of spermatogonia [hamster_oocyte.RNA_seq]

2021-07-13 | GSE164656 | GEO

Golden hamster piRNAs are essential for early development and establishment of spermatogonia [hamster_testis.RNA_seq]

2021-07-13 | GSE164655 | GEO

Golden hamster piRNAs are essential for early development and establishment of spermatogonia [hamster_testis.small_RNAseq]

2021-07-13 | GSE164654 | GEO

SV40 lymphomagenesis in Syrian golden hamsters.

Project description:Simian virus 40 (SV40) isolates differ in oncogenic potential in Syrian golden hamsters following intraperitoneal inoculation. Here we describe the effect of intravenous exposure on tumor induction by SV40. Strains SVCPC (simple regulatory region) and VA45-54(2E) (complex regulatory region) were highly oncogenic following intravenous inoculation, producing a spectrum of tumor types. Three lymphoma cell lines were established; all expressed SV40 T-antigen, were immortalized for growth in culture, and were tumorigenic following transplantation in vivo. New monoclonal antibodies directed against hamster lymphocyte surface antigens are described. The cell lines expressed MHC class II and macrophage markers and were highly phagocytic, indicating a histiocytic origin. Many hamsters that remained tumor-free developed SV40 T-antigen antibodies, suggesting that viral replication occurred. This study shows that route of exposure influences the pathogenesis of SV40-mediated carcinogenesis, that SV40 strain VA45-54(2E) is lymphomagenic in hamsters, that hamster lymphoid cells of histiocytic origin can be transformed in vivo and established in culture, and that reagents to hamster leukocyte differentiation molecules are now available.

| S-EPMC3648995 | biostudies-literature

Generation of transgenic golden Syrian hamsters.

Project description: Not available

| S-EPMC3945892 | biostudies-literature