POLYseq: A poly(β-amino ester)-based vector for multifunctional cellular barcoding.
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ABSTRACT:
SUBMITTER: Dunn A
PROVIDER: S-EPMC8452539 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:Despite evolving stem cell and organoid application of next-generation sequencing (NGS) at single cell level, current techniques in NGS library preparation are restrictive as individual samples within a single library are indistinguishable, necessitating the laborious and costly preparation of distinct libraries for each sample. To combat this challenge, we report the development of a novel poly(ß-amino) ester labeling system synthesized with inexpensive, common reagents, termed POLYseq, capable of efficiently delivering fluorescent molecules or sample-distinguishing DNA barcodes through non-covalent binding enabling rapid creation of custom libraries.
2021-06-22 | GSE163971 | GEO
Project description:POLYseq: A poly(ß-amino ester)-based vector for multifunctional cellular barcoding
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