Identification of contaminating fungal DNA sequences in Zymolyase.
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ABSTRACT:
SUBMITTER: Rimek D
PROVIDER: S-EPMC84574 | biostudies-literature | 1999 Mar
REPOSITORIES: biostudies-literature
Publications
Identification of contaminating fungal DNA sequences in Zymolyase.
Rimek D D Garg A P AP Haas W H WH Kappe R R
Journal of clinical microbiology 19990301 3
When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be free from fungal DNA. ...[more]
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