Unknown

Dataset Information

0

Anoikis resistance in mammary epithelial cells is mediated by semaphorin 7a.


ABSTRACT: Semaphorin-7a (SEMA7A), best known as a neuroimmune molecule, plays a diverse role in many cellular processes and pathologies. Here, we show that SEMA7A promotes anoikis resistance in cultured mammary epithelial cells through integrins and activation of pro-survival kinase AKT, which led us to investigate a role for SEMA7A during postpartum mammary gland involution-a normal developmental process where cells die by anoikis. Our results reveal that SEMA7A is expressed on live mammary epithelial cells during involution, that SEMA7A expression is primarily observed in α6-integrin expressing cells, and that luminal progenitor cells, specifically, are decreased in mammary glands of SEMA7A-/- mice during involution. We further identify a SEMA7A-α6/β1-integrin dependent mechanism of mammosphere formation and chemoresistance in mammary epithelial cells and suggest that this mechanism is relevant for recurrence in breast cancer patients.

SUBMITTER: Rutherford TR 

PROVIDER: S-EPMC8463677 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3459863 | biostudies-other
| S-EPMC6239927 | biostudies-literature
| S-EPMC6338389 | biostudies-literature
| S-EPMC5819760 | biostudies-literature
| S-EPMC2856127 | biostudies-literature
| S-EPMC9935921 | biostudies-literature
| S-EPMC2783190 | biostudies-literature
| S-EPMC4649521 | biostudies-other
| S-EPMC5629104 | biostudies-literature
| S-EPMC6284023 | biostudies-literature