Metal ion fluxes controlling amphibian fertilization.

ABSTRACT: Mammalian oocytes undergo major changes in zinc content and localization to be fertilized, the most striking being the rapid exocytosis of over 10 billion zinc ions in what are known as zinc sparks. Here, we report that fertilization of amphibian Xenopus laevis eggs also initiates a zinc spark that progresses across the cell surface in coordination with dynamic calcium waves. This zinc exocytosis is accompanied by a newly recognized loss of intracellular manganese. Synchrotron-based X-ray fluorescence and analytical electron microscopy reveal that zinc and manganese are sequestered in a system of cortical granules that are abundant at the animal pole. Through electron-nuclear double-resonance studies, we rule out Mn²⁺ complexation with phosphate or nitrogenous ligands in intact

SUBMITTER: Seeler JF 

PROVIDER: S-EPMC8475775 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature