Comparison of Microneutralization and Histo-Blood Group Antigen-Blocking Assays for Functional Norovirus Antibody Detection.

ABSTRACT:

Background

The development of an in vitro cultivation system for human noroviruses allows the measurement of neutralizing antibody levels.

Methods

Serum neutralizing antibody levels were determined using a GII.4/Sydney/2012-like virus in human intestinal enteroids in samples collected before and 4 weeks after administration of an investigational norovirus vaccine and were compared with those measured in histo-blood group antigen (HBGA)-blocking assays.

Results

Neutralizing antibody seroresponses were observed in 71% of 24 vaccinated adults, and antibody levels were highly correlated (r = 0.82, P < .001) with those measured by HBGA blocking.

Conclusions

HBGA-blocking antibodies are a surrogate for neutralization in human noroviruses.

Clinical trials registration

NCT02475278.

SUBMITTER: Atmar RL 

PROVIDER: S-EPMC8483564 | biostudies-literature |

REPOSITORIES: biostudies-literature