A versatile technique for high-resolution three-dimensional imaging of human arterial segments using microcomputed tomography.

ABSTRACT:

Background

Quantitative methods for evaluating microstructure of arterial specimens typically rely on histologic techniques that involve random sampling, which cannot account for the unique spatial distribution of features in three dimensions.

Methods

To overcome this limitation, we demonstrate a nondestructive method for three-dimensional imaging of intact human blood vessels using microcomputed tomography (microCT). Human artery segments were dehydrated and stained in an iodine solution then imaged with a standard laboratory microCT scanner. Image visualization and segmentation was performed using commercially available and open source software.

Results

Staining of cadaveric vessels with iodine enabled clear visualization of the arterial wall with microCT, preserved

SUBMITTER: Robinson ST 

PROVIDER: S-EPMC8489243 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature