Determination of the Near-Atomic Structure of Non-Purified Oligomeric E. coli Proteins
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ABSTRACT: The roughly purified extract of E. coli proteins has been studied by cryoelectron microscopy, the class-sums containing 2D projections of two proteins (β-galactosidase and 2-oxoglutarate dehydrogenase complex catalytic domain (ODC-CD)), identified in an extract by tandem mass spectrometry, have been distinguished. The structures of these proteins have been solved at near-atomic resolution. De novo simulation of the ODC-CD structure yielded an atomic model that revealed differences in the positions of some amino acid residues of the active center, in comparison with the known crystal structures.
SUBMITTER: Pichkur E
PROVIDER: S-EPMC8490847 | biostudies-literature |
REPOSITORIES: biostudies-literature
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