A Multiparametric Assessment of Human Islets Predicts Transplant Outcomes in Diabetic Mice.

ABSTRACT: Prior to transplantation into individuals with type 1 diabetes, in vitro assays are used to evaluate the quality, function and survival of isolated human islets. In addition to the assessments of these parameters in islet, they can be evaluated by multiparametric morphological scoring (0-10 points) and grading (A, B, C, D, and F) based on islet characteristics (shape, border, integrity, single cells, and diameter). However, correlation between the multiparametric assessment and transplantation outcome has not been fully elucidated. In this study, 55 human islet isolations were scored using this multiparametric assessment. The results were correlated with outcomes after transplantation into immunodeficient diabetic mice. In addition, the multiparametric assessment was compared with oxygen c

SUBMITTER: Komatsu H 

PROVIDER: S-EPMC8504220 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature