Unknown

Dataset Information

0

Exendin-4 Pretreatment Attenuates Kainic Acid-Induced Hippocampal Neuronal Death.


ABSTRACT: Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor (GLP-1R) agonist that protects against brain injury. However, little is known about the effect of Ex-4 on kainic acid (KA)-induced seizures and hippocampal cell death. Therefore, this study evaluated the neuroprotective effects of Ex-4 pretreatment in a mouse model of KA-induced seizures. Three days before KA treatment, mice were intraperitoneally injected with Ex-4. We found that Ex-4 pretreatment reversed KA-induced reduction of GLP-1R expression in the hippocampus and attenuated KA-induced seizure score, hippocampal neuronal death, and neuroinflammation. Ex-4 pretreatment also dramatically reduced hippocampal lipocalin-2 protein in KA-treated mice. Furthermore, immunohistochemical studies showed that Ex-4 pretreatment significantly alleviated blood-brain barrier leakage. Finally, Ex-4 pretreatment stimulated hippocampal expression of phosphorylated cyclic adenosine monophosphate (cAMP) response element-binding protein (p-CREB), a known target of GLP-1/GLP-1R signaling. These findings indicate that Ex-4 pretreatment may protect against KA-induced neuronal damage by regulating GLP-1R/CREB-mediated signaling pathways.

SUBMITTER: Ahn YJ 

PROVIDER: S-EPMC8534217 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Exendin-4 Pretreatment Attenuates Kainic Acid-Induced Hippocampal Neuronal Death.

Ahn Yu-Jeong YJ   Shin Hyun-Joo HJ   Jeong Eun-Ae EA   An Hyeong-Seok HS   Lee Jong-Youl JY   Jang Hye-Min HM   Kim Kyung-Eun KE   Lee Jaewoong J   Shin Meong-Cheol MC   Roh Gu-Seob GS  

Cells 20210924 10


Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor (GLP-1R) agonist that protects against brain injury. However, little is known about the effect of Ex-4 on kainic acid (KA)-induced seizures and hippocampal cell death. Therefore, this study evaluated the neuroprotective effects of Ex-4 pretreatment in a mouse model of KA-induced seizures. Three days before KA treatment, mice were intraperitoneally injected with Ex-4. We found that Ex-4 pretreatment reversed KA-induced reduction of GLP-1R exp  ...[more]

Similar Datasets

| S-EPMC2577806 | biostudies-literature
| S-EPMC2998094 | biostudies-literature
| S-EPMC7292224 | biostudies-literature
| S-EPMC6011524 | biostudies-literature
2010-03-01 | GSE10923 | GEO
| S-EPMC5329003 | biostudies-literature
2010-04-06 | E-GEOD-10923 | biostudies-arrayexpress
| S-EPMC7247548 | biostudies-literature
| S-EPMC5220350 | biostudies-literature
| S-EPMC2761958 | biostudies-literature