Dataset Information

Activation of Drosophila melanogaster TRPA1 Isoforms by Citronellal and Menthol.

ABSTRACT: The transient receptor potential ankyrin 1 (TRPA1) cation channels function as broadly-tuned sensors of noxious chemicals in many species. Recent studies identified four functional TRPA1 isoforms in Drosophila melanogaster (dTRPA1(A) to (D)), but their responses to non-electrophilic chemicals are yet to be fully characterized. We determined the behavioral responses of adult flies to the mammalian TRPA1 non-electrophilic activators citronellal and menthol, and characterized the effects of these compounds on all four dTRPA1 channel isoforms using intracellular Ca2+ imaging and whole-cell patch-clamp recordings. Wild type flies avoided citronellal and menthol in an olfactory test and this behavior was reduced in dTrpA1 mutant flies. Both compounds activate all dTRPA1 isoforms in the heterologous expression system HEK293T, with the following sensitivity series: dTRPA1(C) = dTRPA1(D) > dTRPA1(A) ≫ dTRPA1(B) for citronellal and dTRPA1(A) > dTRPA1(D) > dTRPA1(C) > dTRPA1(B) for menthol. dTrpA1 was required for the normal avoidance of Drosophila melanogaster towards citronellal and menthol. All dTRPA1 isoforms are activated by both compounds, but the dTRPA1(B) is consistently the least sensitive. We discuss how these findings may guide further studies on the physiological roles and the structural bases of chemical sensitivity of TRPA1 channels.

SUBMITTER: Boonen B

PROVIDER: S-EPMC8541009 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Publications

Activation of <i>Drosophila melanogaster</i> TRPA1 Isoforms by Citronellal and Menthol.

Boonen Brett B Startek Justyna B JB Milici Alina A López-Requena Alejandro A Beelen Melissa M Callaerts Patrick P Talavera Karel K

International journal of molecular sciences 20211012 20

<h4>Background</h4>The transient receptor potential ankyrin 1 (TRPA1) cation channels function as broadly-tuned sensors of noxious chemicals in many species. Recent studies identified four functional TRPA1 isoforms in <i>Drosophila melanogaster</i> (dTRPA1(A) to (D)), but their responses to non-electrophilic chemicals are yet to be fully characterized.<h4>Methods</h4>We determined the behavioral responses of adult flies to the mammalian TRPA1 non-electrophilic activators citronellal and menthol, ...[more]

PMID: 34681657

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Project description:The environment to which insects have been exposed as larvae and adults can affect subsequent behaviors, such as mating, oviposition, food preference or fitness. Experience can change female preference for oviposition, particularly in phytophagous insects. In Drosophila melanogaster, females avoid laying eggs on menthol rich-food when given the choice. Exposure to menthol during larval development reduces this aversion. However, this observation was not reproduced in the following generation. Recently, we have shown that oviposition-site preference (OSP) differs between wild-type D. melanogaster lines freely or forcibly exposed to menthol. After 12 generations, menthol "forced" lines still exhibit a persistent aversion to menthol whereas 'free-choice' lines show a decreased aversion for menthol rich-food. Here, we compare courtship behavior, mating and female fecundity in "forced" and "free-choice" lines, raised either on menthol rich-food (Menthol-lines) or on menthol-free food (Plain-lines). "Forced" males did not discriminate between decapitated virgin females of the two lines. They courted and mated with intact females of both "forced" lines in a comparable rate. However "forced" M-line males did mate significantly more rapidly with "forced" M-line females. In the "free-choice" procedure, P-line males show a similar pattern as "forced" males for discrimination ability and courtship. M-line males courted significantly more M-line females. Both 'free-choice' lines males mated significantly more with females of their own line. Female fecundity was assessed during 10 days in 'free-choice' lines. Menthol-line females laid more eggs during the first 4 days than female Plain-lines and parental control-line. The total number of eggs laid during the first 10 days of female adult life is comparable in M-line and parental control line. However, Menthol-line females laid eggs earlier than both parental control and Plain-lines. Our findings show that in D. melanogaster, as for OSP, mating and fecundity are more rapidly influenced when flies have a choice between alternative resources compared to flies permanently exposed to menthol.

Dataset Information

Activation of Drosophila melanogaster TRPA1 Isoforms by Citronellal and Menthol.

Publications

Activation of <i>Drosophila melanogaster</i> TRPA1 Isoforms by Citronellal and Menthol.

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