Mass production of human airway-like neutrophils via transmigration in an organotypic model of human airways

ABSTRACT: Summary Neutrophils are difficult to study, particularly in tissues, due to their short half-life and propensity for activation. We describe an organotypic airway model that uses patient airway fluid to enable the transmigration of blood neutrophils to acquire an airway-like phenotype in order to better understand their contribution to airway diseases. In particular, we showcase how conditioned neutrophils modulate their bacteria-killing abilities. For complete details on the use and execution of this protocol, please refer to Margaroli et al. (2021). Graphical abstract Highlights • A lung organotypic model allows epithelial cells to be grown at an air-liquid interface• Primary blood neutrophils (and other leukocytes) can transmigrate across the epithelium• Patient airway fluid added apically conditions transmigrating cells, which mirrors the in vivo state• Drugs can be used to understand underlying mechanisms driving disease Neutrophils are difficult to study, particularly in tissues, due to their short half-life and propensity for activation. We describe an organotypic airway model that uses patient airway fluid to enable the transmigration of blood neutrophils to acquire an airway-like phenotype in order to better understand their contribution to airway diseases. In particular, we showcase how conditioned neutrophils modulate their bacteria-killing abilities.

SUBMITTER: Dobosh B 

PROVIDER: S-EPMC8551927 | biostudies-literature |

REPOSITORIES: biostudies-literature