Ontology highlight
ABSTRACT:
SUBMITTER: Matsuzaki Y
PROVIDER: S-EPMC8563947 | biostudies-literature | 2021 Nov
REPOSITORIES: biostudies-literature
Scientific reports 20211102 1
Optimisation of protein binders relies on laborious screening processes. Investigation of sequence-function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed peptide barcoding, a high-throughput approach for accurate investigation of sequence-function relationships of hundreds of protein binders at once. Our approach is based on combining the generation of a mutagenised nanobody library fused with unique peptide barcod ...[more]