Unknown

Dataset Information

0

Ca2+ imbalance caused by ERdj5 deletion affects mitochondrial fragmentation


ABSTRACT: The endoplasmic reticulum (ER) is the organelle responsible for the folding of secretory/membrane proteins and acts as a dynamic calcium ion (Ca2+) store involved in various cellular signalling pathways. Previously, we reported that the ER-resident disulfide reductase ERdj5 is involved in the ER-associated degradation (ERAD) of misfolded proteins in the ER and the activation of SERCA2b, a Ca2+ pump on the ER membrane. These results highlighted the importance of the regulation of redox activity in both Ca2+ and protein homeostasis in the ER. Here, we show that the deletion of ERdj5 causes an imbalance in intracellular Ca2+ homeostasis, the activation of Drp1, a cytosolic GTPase involved in mitochondrial fission, and finally the aberrant fragmentation of mitochondria, which affects cell viability as well as phenotype with features of cellular senescence. Thus, ERdj5-mediated regulation of intracellular Ca2+ is essential for the maintenance of mitochondrial homeostasis involved in cellular senescence.

SUBMITTER: Yamashita R 

PROVIDER: S-EPMC8563984 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7261923 | biostudies-literature
| S-EPMC10424509 | biostudies-literature
| S-EPMC5423111 | biostudies-literature
| S-EPMC5403897 | biostudies-literature
| S-EPMC8353469 | biostudies-literature
| S-EPMC6640624 | biostudies-literature
| S-EPMC8496184 | biostudies-literature
| S-EPMC7116774 | biostudies-literature
| S-EPMC5293110 | biostudies-literature
| S-EPMC6788349 | biostudies-literature