SMYD2-mediated TRAF2 methylation promotes the NF-κB signaling pathways in inflammatory diseases.

ABSTRACT:

Background

The methylation of lysine residues has been involved in the multiple biological and diseases processes. Recently, some particular non-histone proteins have been elucidated to be methylated by SMYD2, a SET and MYND domain protein with lysine methyltransferase activity.

Methods

SMYD2 was evaluated in synovial tissue and cells derived from rheumatoid arthritis patients. We confirmed TRAF2 could be methylated by SMYD2 using Mass spectrometry, pull-down, immunoprecipitation, methyltransferase assay, ubiquitination assay, luciferase reporter assays, and western blot analyses. Using loss- and gain-of function studies, we explored the biological functions of SMYD2 in vitro and in vivo. Using acute and chronic inflammation with different mice models to determine the impact

SUBMITTER: Wu W 

PROVIDER: S-EPMC8567046 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature