Dataset Information

Detecting Plant Stress Using Thermal and Optical Imagery From an Unoccupied Aerial Vehicle

ABSTRACT: Soil and water salinization has global impact on the sustainability of agricultural production, affecting the health and condition of staple crops and reducing potential yields. Identifying or developing salt-tolerant varieties of commercial crops is a potential pathway to enhance food and water security and deliver on the global demand for an increase in food supplies. Our study focuses on a phenotyping experiment that was designed to establish the influence of salinity stress on a diversity panel of the wild tomato species, Solanum pimpinellifolium. Here, we explore how unoccupied aerial vehicles (UAVs) equipped with both an optical and thermal infrared camera can be used to map and monitor plant temperature (Tp) changes in response to applied salinity stress. An object-based image analysis approach was developed to delineate individual tomato plants, while a green–red vegetation index derived from calibrated red, green, and blue (RGB) optical data allowed the discrimination of vegetation from the soil background. Tp was retrieved simultaneously from the co-mounted thermal camera, with Tp deviation from the ambient temperature and its change across time used as a potential indication of stress. Results showed that Tp differences between salt-treated and control plants were detectable across the five separate UAV campaigns undertaken during the field experiment. Using a simple statistical approach, we show that crop water stress index values greater than 0.36 indicated conditions of plant stress. The optimum period to collect UAV-based Tp for identifying plant stress was found between fruit formation and ripening. Preliminary results also indicate that UAV-based Tp may be used to detect plant stress before it is visually apparent, although further research with more frequent image collections and field observations is required. Our findings provide a tool to accelerate field phenotyping to identify salt-resistant germplasm and may allow farmers to alleviate yield losses through early detection of plant stress via management interventions.

SUBMITTER: Stutsel B

PROVIDER: S-EPMC8579776 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BackgroundThe objective of this study is twofold. First, ascertain the important variables that predict tomato yields from plant height (PH) and vegetation index (VI) maps. The maps were derived from images taken by unmanned aerial vehicles (UAVs). Second, examine the accuracy of predictions of tomato fresh shoot masses (SM), fruit weights (FW), and the number of fruits (FN) from multiple machine learning algorithms using selected variable sets. To realize our objective, ultra-high-resolution RGB and multispectral images were collected by a UAV on ten days in 2020's tomato growing season. From these images, 756 total variables, including first- (e.g., average, standard deviation, skewness, range, and maximum) and second-order (e.g., gray-level co-occurrence matrix features and growth rates of PH and VIs) statistics for each plant, were extracted. Several selection algorithms (i.e., Boruta, DALEX, genetic algorithm, least absolute shrinkage and selection operator, and recursive feature elimination) were used to select the variable sets useful for predicting SM, FW, and FN. Random forests, ridge regressions, and support vector machines were used to predict the yield using the top five selected variable sets.ResultsFirst-order statistics of PH and VIs collected during the early to mid-fruit formation periods, about one month prior to harvest, were important variables for predicting SM. Similar to the case for SM, variables collected approximately one month prior to harvest were important for predicting FW and FN. Furthermore, variables related to PH were unimportant for prediction. Compared with predictions obtained using only first-order statistics, those obtained using the second-order statistics of VIs were more accurate for FW and FN. The prediction accuracy of SM, FW, and FN by models constructed from all variables (rRMSE = 8.8-28.1%) was better than that from first-order statistics (rRMSE = 10.0-50.1%).ConclusionsIn addition to basic statistics (e.g., average and standard deviation), we derived second-order statistics of PH and VIs at the plant level using the ultra-high resolution UAV images. Our findings indicated that our variable selection method reduced the number variables needed for tomato yield prediction, improving the efficiency of phenotypic data collection and assisting with the selection of high-yield lines within breeding programs.

Project description:Agricultural subsurface drainage systems are commonly installed on farmland to remove the excess water from poorly drained soils. Conventional methods for drainage mapping such as tile probes and trenching equipment are laborious, cause pipe damage, and are often inefficient to apply at large spatial scales. Knowledge of locations of an existing drainage network is crucial to understand the increased leaching and offsite release of drainage discharge and to retrofit the new drain lines within the existing drainage system. Recent technological developments in non-destructive techniques might provide a potential alternative solution. The objective of this study was to determine the suitability of unmanned aerial vehicle (UAV) imagery collected using three different cameras (visible-color, multispectral, and thermal infrared) and ground penetrating radar (GPR) for subsurface drainage mapping. Both the techniques are complementary in terms of their usage, applicability, and the properties they measure and were applied at four different sites in the Midwest USA. At Site-1, both the UAV imagery and GPR were equally successful across the entire field, while at Site-2, the UAV imagery was successful in one section of the field, and GPR proved to be useful in the other section where the UAV imagery failed to capture the drainage pipes' location. At Site-3, less to no success was observed in finding the drain lines using UAV imagery captured on bare ground conditions, whereas good success was achieved using GPR. Conversely, at Site-4, the UAV imagery was successful and GPR failed to capture the drainage pipes' location. Although UAV imagery seems to be an attractive solution for mapping agricultural subsurface drainage systems as it is cost-effective and can cover large field areas, the results suggest the usefulness of GPR to complement the former as both a mapping and validation technique. Hence, this case study compares and contrasts the suitability of both the methods, provides guidance on the optimal survey timing, and recommends their combined usage given both the technologies are available to deploy for drainage mapping purposes.

Project description:Non-forest ecosystems, dominated by shrubs, grasses and herbaceous plants, provide ecosystem services including carbon sequestration and forage for grazing, and are highly sensitive to climatic changes. Yet these ecosystems are poorly represented in remotely sensed biomass products and are undersampled by in situ monitoring. Current global change threats emphasize the need for new tools to capture biomass change in non-forest ecosystems at appropriate scales. Here we developed and deployed a new protocol for photogrammetric height using unoccupied aerial vehicle (UAV) images to test its capability for delivering standardized measurements of biomass across a globally distributed field experiment. We assessed whether canopy height inferred from UAV photogrammetry allows the prediction of aboveground biomass (AGB) across low-stature plant species by conducting 38 photogrammetric surveys over 741 harvested plots to sample 50 species. We found mean canopy height was strongly predictive of AGB across species, with a median adjusted R 2 of 0.87 (ranging from 0.46 to 0.99) and median prediction error from leave-one-out cross-validation of 3.9%. Biomass per-unit-of-height was similar within but different among, plant functional types. We found that photogrammetric reconstructions of canopy height were sensitive to wind speed but not sun elevation during surveys. We demonstrated that our photogrammetric approach produced generalizable measurements across growth forms and environmental settings and yielded accuracies as good as those obtained from in situ approaches. We demonstrate that using a standardized approach for UAV photogrammetry can deliver accurate AGB estimates across a wide range of dynamic and heterogeneous ecosystems. Many academic and land management institutions have the technical capacity to deploy these approaches over extents of 1-10 ha-1. Photogrammetric approaches could provide much-needed information required to calibrate and validate the vegetation models and satellite-derived biomass products that are essential to understand vulnerable and understudied non-forested ecosystems around the globe.

Project description:Plant nitrogen concentration (PNC) is a critical indicator of N status for crops, and can be used for N nutrition diagnosis and management. This work aims to explore the potential of multispectral imagery from unmanned aerial vehicle (UAV) for PNC estimation and improve the estimation accuracy with hyperspectral data collected in the field with a hyperspectral radiometer. In this study we combined selected vegetation indices (VIs) and texture information to estimate PNC in rice. The VIs were calculated from ground and aerial platforms and the texture information was obtained from UAV-based multispectral imagery. Two consecutive years (2015 & 2016) of experiments were conducted, involving different N rates, planting densities and rice cultivars. Both UAV flights and ground spectral measurements were taken along with destructive samplings at critical growth stages of rice (Oryza sativa L.). After UAV imagery preprocessing, both VIs and texture measurements were calculated. Then the optimal normalized difference texture index (NDTI) from UAV imagery was determined for separated stage groups and the entire season. Results demonstrated that aerial VIs performed well only for pre-heading stages (R2 = 0.52-0.70), and photochemical reflectance index and blue N index from ground (PRIg and BNIg) performed consistently well across all growth stages (R2 = 0.48-0.65 and 0.39-0.68). Most texture measurements were weakly related to PNC, but the optimal NDTIs could explain 61 and 51% variability of PNC for separated stage groups and entire season, respectively. Moreover, stepwise multiple linear regression (SMLR) models combining aerial VIs and NDTIs did not significantly improve the accuracy of PNC estimation, while models composed of BNIg and optimal NDTIs exhibited significant improvement for PNC estimation across all growth stages. Therefore, the integration of ground-based narrow band spectral indices with UAV-based textural information might be a promising technique in crop growth monitoring.

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Detecting Plant Stress Using Thermal and Optical Imagery From an Unoccupied Aerial Vehicle

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