A programmable hierarchical-responsive nanoCRISPR elicits robust activation of endogenous target to treat cancer.

ABSTRACT: Despite promising progress of cancer gene therapy made, these therapeutics were still limited by the diversity of gene sizes and types. CRISPR/dCas9 mediated activation of tumor endogenous gene has shown great potential to surmount hinders of genetic varieties during the process of cancer gene therapy. However, the blood interference along with complicated tumor extra/intracellular microenvironment substantially compromise the performance of CRISPR/dCas9-based therapeutics in vivo. Methods: In this study, we constructed a programmable hierarchical-responsive nanoCRISPR (PICASSO) that can achieve sequential responses to the multiple physiological barriers in vivo. The core-shell structure endows PICASSO with long blood circulation capacity and tumor target accumulation

SUBMITTER: Liu C 

PROVIDER: S-EPMC8581410 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature