Unknown

Dataset Information

0

An anaphase surveillance mechanism prevents micronuclei formation from frequent chromosome segregation errors.


ABSTRACT: Micronuclei are a hallmark of cancer and several other human disorders. Recently, micronuclei were implicated in chromothripsis, a series of massive genomic rearrangements that may drive tumor evolution and progression. Here, we show that Aurora B kinase mediates a surveillance mechanism that integrates error correction during anaphase with spatial control of nuclear envelope reassembly to prevent micronuclei formation. Using high-resolution live-cell imaging of human cancer and non-cancer cells, we uncover that anaphase lagging chromosomes are more frequent than previously anticipated, yet they rarely form micronuclei. Micronuclei formation from anaphase lagging chromosomes is prevented by a midzone-based Aurora B phosphorylation gradient that stabilizes kinetochore-microtubule attachments and assists spindle forces required for anaphase error correction while delaying nuclear envelope reassembly on lagging chromosomes, independently of microtubule density. We propose that a midzone-based Aurora B phosphorylation gradient actively monitors and corrects frequent chromosome segregation errors to prevent micronuclei formation during human cell division.

SUBMITTER: Orr B 

PROVIDER: S-EPMC8595644 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7198627 | biostudies-literature
| S-EPMC6051344 | biostudies-literature
| S-EPMC2586334 | biostudies-literature
| S-EPMC2148171 | biostudies-literature
| S-EPMC9302971 | biostudies-literature
| S-EPMC6509349 | biostudies-literature
| S-EPMC4038388 | biostudies-literature
| S-EPMC3410419 | biostudies-literature
| S-EPMC5679441 | biostudies-literature
| S-EPMC4752446 | biostudies-literature