Ontology highlight
ABSTRACT:
SUBMITTER: Pereira AHM
PROVIDER: S-EPMC8599495 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Pereira Ana Helena Macedo AHM Cardoso Alisson Campos AC Franchini Kleber Gomes KG
STAR protocols 20211112 4
Isolation and culture of ventricular cardiomyocytes from neonatal rats (NRVMs) is a powerful model to study neonatal cardiac development, cell cycle regulation, and cardiac physiology and pathology <i>in vitro</i>. Here, we present our modified enzymatic digestion protocol followed by two-step discontinuous Percoll gradient centrifugation to isolate a high yield of viable ventricular cardiomyocytes from neonatal rats. Finally, here we describe an immunostaining protocol for cytosolic and nuclear ...[more]