Responses of CD27⁺ CD38⁺ plasmablasts, and CD24^hi CD27^hi and CD24^hi CD38^hi regulatory B cells during primary dengue virus 2 infection.

ABSTRACT:

Background

Humoral immunity is thought to play a central role in mediating the immunopathogenesis of dengue virus (DENV) infection; however, the B-cell responses elicited by primary DENV2 infection are incompletely understood. Follicular helper T cells (Tfh) are important to promote B-cell activation and differentiation.

Methods

The present study analyzed the detailed dynamic changes of circulating B-cell subsets and Tfh (cTfh) using flow cytometry to explore their responses to DENV2 infection.

Results

Thirty-six patients with DENV2 and 21 healthy individuals were included. The results showed that CD27⁺ CD38⁺ plasmablasts emerged after DENV2 infection, and correlated with CXCR5⁺ PD-1⁺ or CXCR5⁺ ICOS⁺ PD-1⁺ cTfh, which increased after DENV2 infection, and correlated with DENV2 RNA viral loads. Significantly low levels of CD27^- naïve B cells, and CD24^hi CD27^hi and CD24^hi CD38^hi regulatory B cells (Breg) were observed after DENV2 infection, which correlated negatively with CXCR5⁺ PD-1⁺ or CXCR5⁺ ICOS⁺ PD-1⁺ cTfh cells.

Conclusion

Overall, these results provide insights into the DENV2-elicited B-cell response and revealed previously unidentified CD24^hi CD27^hi and CD24^hi CD38^hi Breg responses to DENV2 infection.

SUBMITTER: Lei C 

PROVIDER: S-EPMC8605120 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature