P53-Mediated Radiosensitization of ¹⁷⁷Lu-DOTATATE in Neuroblastoma Tumor Spheroids.

ABSTRACT: p53 is involved in DNA damage response and is an exciting target for radiosensitization in cancer. Targeted radionuclide therapy against somatostatin receptors with ¹⁷⁷Lu-DOTATATE is currently being explored as a treatment for neuroblastoma. The aim of this study was to investigate the novel p53-stabilizing peptide VIP116 in neuroblastoma, both as monotherapy and together with ¹⁷⁷Lu-DOTATATE. Five neuroblastoma cell lines, including two patient-derived xenograft (PDX) lines, were characterized in monolayer cultures. Four out of five were positive for ¹⁷⁷Lu-DOTATATE uptake. IC₅₀ values after VIP116 treatments correlated with p53 status, ranging between 2.8-238.2 μM. IMR-32 and PDX lines LU-NB-1 and LU-NB-2 were then cultured as multicellular tumor

SUBMITTER: Lundsten S 

PROVIDER: S-EPMC8615514 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature