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Room-temperature dynamic nuclear polarization enhanced NMR spectroscopy of small biological molecules in water.


ABSTRACT: Nuclear magnetic resonance (NMR) spectroscopy is a powerful and popular technique for probing the molecular structures, dynamics and chemical properties. However the conventional NMR spectroscopy is bottlenecked by its low sensitivity. Dynamic nuclear polarization (DNP) boosts NMR sensitivity by orders of magnitude and resolves this limitation. In liquid-state this revolutionizing technique has been restricted to a few specific non-biological model molecules in organic solvents. Here we show that the carbon polarization in small biological molecules, including carbohydrates and amino acids, can be enhanced sizably by in situ Overhauser DNP (ODNP) in water at room temperature and at high magnetic field. An observed connection between ODNP 13C enhancement factor and paramagnetic 13C NMR shift has led to the exploration of biologically relevant heterocyclic compound indole. The QM/MM MD simulation underscores the dynamics of intermolecular hydrogen bonds as the driving force for the scalar ODNP in a long-living radical-substrate complex. Our work reconciles results obtained by DNP spectroscopy, paramagnetic NMR and computational chemistry and provides new mechanistic insights into the high-field scalar ODNP.

SUBMITTER: Dai D 

PROVIDER: S-EPMC8616939 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Room-temperature dynamic nuclear polarization enhanced NMR spectroscopy of small biological molecules in water.

Dai Danhua D   Wang Xianwei X   Liu Yiwei Y   Yang Xiao-Liang XL   Glaubitz Clemens C   Denysenkov Vasyl V   He Xiao X   Prisner Thomas T   Mao Jiafei J  

Nature communications 20211125 1


Nuclear magnetic resonance (NMR) spectroscopy is a powerful and popular technique for probing the molecular structures, dynamics and chemical properties. However the conventional NMR spectroscopy is bottlenecked by its low sensitivity. Dynamic nuclear polarization (DNP) boosts NMR sensitivity by orders of magnitude and resolves this limitation. In liquid-state this revolutionizing technique has been restricted to a few specific non-biological model molecules in organic solvents. Here we show tha  ...[more]

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