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Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara(-) phenotypes.

ABSTRACT: Dot blot hybridization and PCR amplification of 14 Ara(+) and 8 Ara(-) Burkholderia pseudomallei strains showed that type III secretion (TTS) genes were present in all the Ara(-) strains but absent from all but one of the Ara(+) strains. The link between TTS genes and an Ara(-) phenotype suggests a role for TTS in virulence.

SUBMITTER: Winstanley C

PROVIDER: S-EPMC86234 | biostudies-literature | 2000 Feb

REPOSITORIES: biostudies-literature

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Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara(-) phenotypes.

Winstanley C C Hart C A CA

Journal of clinical microbiology 20000201 2

Dot blot hybridization and PCR amplification of 14 Ara(+) and 8 Ara(-) Burkholderia pseudomallei strains showed that type III secretion (TTS) genes were present in all the Ara(-) strains but absent from all but one of the Ara(+) strains. The link between TTS genes and an Ara(-) phenotype suggests a role for TTS in virulence. ...[more]

PMID: 10655407

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Eukaryotic pathways targeted by the type III secretion system effector protein, BipC, involved in the intracellular lifecycle of Burkholderia pseudomallei.

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Project description:Melioidosis, caused by the soil-dwelling bacterium Burkholderia pseudomallei, is predicted to be endemic in Nigeria but is only occasionally reported. This report documents the systematic identification of the presence of B. pseudomallei and B. thailandensis in the soil across multiple states in Nigeria.

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