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Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin

ABSTRACT: Highlights • EMCP is fractionated by ion-exchange and gel permeation chromatographies.• EMCP fractions contain glucan backbone and different saccharides as side chains.• RG-II domain may weaken the binding strength between EMCP fractions and Gal-3.• EMCP-3p and EMCP-2p exhibit strong cytotoxicity against MCF-7 and A549 cell lines. Modified citrus pectin (MCP), a commercially available dietary supplement prepared from citrus pectin, contains several different polysaccharide domains, but its primary chemical structure and the binding epitopes that antagonize galectin-3 function remain unclear. In this study, five fractions were isolated from MCP after endo-polygalacturonase degradation (EMCP) and a combination of DEAE-cellulose and Sepharose CL-6B or Sephadex G-75 chromatography. Their primary structures, abilities to inhibit galectin-3-mediated hemagglutination, and antiproliferation activities on MCF-7 and A549 cell lines were studied. Results showed that EMCP-3p, one of the five fractions, was composed of Glc (89.8%), Gal (3.8%), Ara (3.1%), GalA (1.1%), Man (0.9%), and Rha (1.3%) with an average molecular weight of 88.4 KDa, which had the most substantial degree of galectin-3 inhibition with an MIC of 31.25 μg/mL, and it exhibited remarkable cytotoxicity against MCF-7 (36.7%) and A549 (57.4%) cell lines. These results provide new insight into the structure–function relationships of EMCP-derived polysaccharides.

SUBMITTER: Zhang T

PROVIDER: S-EPMC8628203 | biostudies-literature |

REPOSITORIES: biostudies-literature

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