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Intelligent demethylase-driven DNAzyme sensor for highly reliable metal-ion imaging in living cells.


ABSTRACT: The accurate intracellular imaging of metal ions requires an exquisite site-specific activation of metal-ion sensors, for which the pervasive epigenetic regulation strategy can serve as an ideal alternative thanks to its orthogonal control feature and endogenous cell/tissue-specific expression pattern. Herein, a simple yet versatile demethylation strategy was proposed for on-site repairing-to-activating the metal-ion-targeting DNAzyme and for achieving the accurate site-specific imaging of metal ions in live cells. This endogenous epigenetic demethylation-regulating DNAzyme system was prepared by modifying the DNAzyme with an m6A methylation group that incapacitates the DNAzyme probe, thus eliminating possible off-site signal leakage, while the cell-specific demethylase-mediated removal of methylation modification could efficiently restore the initial catalytic DNAzyme for sensing metal ions, thus allowing a high-contrast bioimaging in live cells. This epigenetic repair-to-activate DNAzyme strategy may facilitate the robust visualization of disease-specific biomarkers for in-depth exploration of their biological functions.

SUBMITTER: Hong C 

PROVIDER: S-EPMC8635203 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Intelligent demethylase-driven DNAzyme sensor for highly reliable metal-ion imaging in living cells.

Hong Chen C   Wang Qing Q   Chen Yingying Y   Gao Yuhui Y   Shang Jinhua J   Weng Xiaocheng X   Liu Xiaoqing X   Wang Fuan F  

Chemical science 20211029 46


The accurate intracellular imaging of metal ions requires an exquisite site-specific activation of metal-ion sensors, for which the pervasive epigenetic regulation strategy can serve as an ideal alternative thanks to its orthogonal control feature and endogenous cell/tissue-specific expression pattern. Herein, a simple yet versatile demethylation strategy was proposed for on-site repairing-to-activating the metal-ion-targeting DNAzyme and for achieving the accurate site-specific imaging of metal  ...[more]

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