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Genome Sequence of the Thermophilic Soil Bacterium Ureibacillus terrenus ATCC BAA-384^T.

ABSTRACT: Ureibacillus terrenus TH9A^T (=ATCC BAA-384^T) was isolated from uncultivated soil in Italy in 1995. We present a draft genome sequence for the type strain, with a predicted genome length of 2,936,851 bp, containing 2,766 protein-coding genes, 82 RNA genes, and 5 CRISPR arrays, with a G+C content of 42.5%.

SUBMITTER: Simoes Junior M

PROVIDER: S-EPMC8638579 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BACKGROUND:Rhodococci are industrially important soil-dwelling Gram-positive bacteria that are well known for both nitrile hydrolysis and oxidative metabolism of aromatics. Rhodococcus rhodochrous ATCC BAA-870 is capable of metabolising a wide range of aliphatic and aromatic nitriles and amides. The genome of the organism was sequenced and analysed in order to better understand this whole cell biocatalyst. RESULTS:The genome of R. rhodochrous ATCC BAA-870 is the first Rhodococcus genome fully sequenced using Nanopore sequencing. The circular genome contains 5.9 megabase pairs (Mbp) and includes a 0.53 Mbp linear plasmid, that together encode 7548 predicted protein sequences according to BASys annotation, and 5535 predicted protein sequences according to RAST annotation. The genome contains numerous oxidoreductases, 15 identified antibiotic and secondary metabolite gene clusters, several terpene and nonribosomal peptide synthetase clusters, as well as 6 putative clusters of unknown type. The 0.53 Mbp plasmid encodes 677 predicted genes and contains the nitrile converting gene cluster, including a nitrilase, a low molecular weight nitrile hydratase, and an enantioselective amidase. Although there are fewer biotechnologically relevant enzymes compared to those found in rhodococci with larger genomes, such as the well-known Rhodococcus jostii RHA1, the abundance of transporters in combination with the myriad of enzymes found in strain BAA-870 might make it more suitable for use in industrially relevant processes than other rhodococci. CONCLUSIONS:The sequence and comprehensive description of the R. rhodochrous ATCC BAA-870 genome will facilitate the additional exploitation of rhodococci for biotechnological applications, as well as enable further characterisation of this model organism. The genome encodes a wide range of enzymes, many with unknown substrate specificities supporting potential applications in biotechnology, including nitrilases, nitrile hydratase, monooxygenases, cytochrome P450s, reductases, proteases, lipases, and transaminases.

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Genome Sequence of the Thermophilic Soil Bacterium Ureibacillus terrenus ATCC BAA-384^T.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Genome Sequence of the Thermophilic Soil Bacterium Ureibacillus terrenus ATCC BAA-384T.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Genome Sequence of the Thermophilic Soil Bacterium Ureibacillus terrenus ATCC BAA-384^T.