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Draft Genome Sequence of Xanthobacter aminoxidans ATCC BAA-299^T.

ABSTRACT: Xanthobacter aminoxidans is a Gram-negative pleomorphic and diazotrophic Knallgas bacillus that undergoes asymmetric budding of V-shaped branched cells during cell division. Like other Xanthobacter spp., cells are yellow from production of zeaxanthine dirhamnoside. We sequenced strain 14a^T (= ATCC BAA-299^T) and report a genome size of 5,829,486 bp with a G+C content of 67.9%.

SUBMITTER: Siddiqui FA

PROVIDER: S-EPMC9387252 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Draft Genome Sequence of Xanthobacter aminoxidans ATCC BAA-299T.

Siddiqui Fardeen A FA Trudel Shoshana S Goen Abigail E AE Fatima Syeda A SA MacLea Kyle S KS

Microbiology resource announcements 20220713 8

Xanthobacter aminoxidans is a Gram-negative pleomorphic and diazotrophic Knallgas bacillus that undergoes asymmetric budding of V-shaped branched cells during cell division. Like other Xanthobacter spp., cells are yellow from production of zeaxanthine dirhamnoside. We sequenced strain 14aT (= ATCC BAA-299T) and report a genome size of 5,829,486 bp with a G+C content of 67.9%. ...[more]

PMID: 35862911

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Project description:BackgroundRhodococci are industrially important soil-dwelling Gram-positive bacteria that are well known for both nitrile hydrolysis and oxidative metabolism of aromatics. Rhodococcus rhodochrous ATCC BAA-870 is capable of metabolising a wide range of aliphatic and aromatic nitriles and amides. The genome of the organism was sequenced and analysed in order to better understand this whole cell biocatalyst.ResultsThe genome of R. rhodochrous ATCC BAA-870 is the first Rhodococcus genome fully sequenced using Nanopore sequencing. The circular genome contains 5.9 megabase pairs (Mbp) and includes a 0.53 Mbp linear plasmid, that together encode 7548 predicted protein sequences according to BASys annotation, and 5535 predicted protein sequences according to RAST annotation. The genome contains numerous oxidoreductases, 15 identified antibiotic and secondary metabolite gene clusters, several terpene and nonribosomal peptide synthetase clusters, as well as 6 putative clusters of unknown type. The 0.53 Mbp plasmid encodes 677 predicted genes and contains the nitrile converting gene cluster, including a nitrilase, a low molecular weight nitrile hydratase, and an enantioselective amidase. Although there are fewer biotechnologically relevant enzymes compared to those found in rhodococci with larger genomes, such as the well-known Rhodococcus jostii RHA1, the abundance of transporters in combination with the myriad of enzymes found in strain BAA-870 might make it more suitable for use in industrially relevant processes than other rhodococci.ConclusionsThe sequence and comprehensive description of the R. rhodochrous ATCC BAA-870 genome will facilitate the additional exploitation of rhodococci for biotechnological applications, as well as enable further characterisation of this model organism. The genome encodes a wide range of enzymes, many with unknown substrate specificities supporting potential applications in biotechnology, including nitrilases, nitrile hydratase, monooxygenases, cytochrome P450s, reductases, proteases, lipases, and transaminases.

Dataset Information

Draft Genome Sequence of Xanthobacter aminoxidans ATCC BAA-299^T.

Publications

Draft Genome Sequence of Xanthobacter aminoxidans ATCC BAA-299<sup>T</sup>.

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