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Site-Selective Functionalization of Methionine Residues via Photoredox Catalysis.


ABSTRACT: Bioconjugation technologies have revolutionized the practice of biology and medicine by allowing access to novel biomolecular scaffolds. New methods for residue-selective bioconjugation are highly sought to expand the toolbox for a variety of bioconjugation applications. Herein we report a site-selective methionine bioconjugation protocol that uses photoexcited lumiflavin to generate open-shell intermediates. This reduction-potential-gated strategy enables access to residues unavailable with traditional nucleophilicity-based conjugation methods. To demonstrate the versatility and robustness of this new protocol, we have modified various proteins and further utilized this functional handle to append diverse biological payloads.

SUBMITTER: Kim J 

PROVIDER: S-EPMC8647115 | biostudies-literature |

REPOSITORIES: biostudies-literature

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