Unknown

Dataset Information

0

Comprehensive Analyses of Type 1 Diabetes Ketosis- or Ketoacidosis-Related Genes in Activated CD56+CD16+ NK Cells.

ABSTRACT:

Objectives

Alterations in natural killer (NK) cells activity cause damage to pancreatic islets in type 1 diabetes mellitus (T1DM). The aim of this study is to identify T1DM ketosis- or ketoacidosis-related genes in activated CD56+CD16+ NK cells.

Methods

Microarray datasets were downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were analyzed using the GEO2R tool. Enrichment analyses were performed using Metascape online database and GSEA software. Cell-specific gene co-expression network was built using NetworkAnalyst tools. Cytoscape software was used to identify hub genes and construct co-expressed networks. Target miRNAs were predicted based on the DIANA-micro T, miRDB, and miRWalk online databases.

Results

A total of 70 DEGs were identified between T1DM patients recovered from ketosis or ketoacidosis and healthy control blood samples in GSE44314. Among the DEGs, 10 hub genes were screened out. The mature NK cell-specific gene co-expression network for DEGs in T1DM was built using NetworkAnalyst tools. DEGs between activated CD56+CD16+ NK cells and CD56brightCD16- NK cells were identified from GSE1511. After intersection, 13 overlapping genes between GSE44314 and GSE1511 microarray datasets were screened out, in which 7 hub genes were identified. Additionally, 59 target miRNAs were predicted according to the 7 hub genes. After validating with the exosome miRNA expression profile dataset of GSE97123, seven differentially expressed miRNAs (DEmiRNAs) in plasma-derived exosome were selected. Finally, a mRNA-miRNA network was constructed, which was involved in the T1DM ketosis or ketoacidosis process.

Conclusion

This work identified seven hub genes in activated CD56+CD16+ NK cells and seven miRNAs in plasma-derived exosome as potential predictors of T1DM ketoacidosis, which provided a novel insight for the pathogenesis at the transcriptome level.

SUBMITTER: Shi R 

PROVIDER: S-EPMC8656236 | biostudies-literature |

REPOSITORIES: biostudies-literature

Json Xml

Similar Datasets

Unknown Depletion but Activation of CD56<sup>dim</sup>CD16<sup>+</sup> NK Cells in Acute Infection with Severe Fever with Thrombocytopenia Syndrome Virus.
| S-EPMC7736421 | biostudies-literature
Transcriptomics Gene expression profile of CD56dimCD16+, CD56brightCD16- and activated CD56+CD16+ human NK subsets
2004-08-02 | GSE1511 | GEO
Unknown Reduced frequency of cytotoxic CD56<sup>dim</sup> CD16<sup>+</sup> NK cells leads to impaired antibody-dependent degranulation in EBV-positive classical Hodgkin lymphoma.
| S-EPMC8738354 | biostudies-literature
Unknown Loss of CCR7 expression on CD56(bright) NK cells is associated with a CD56(dim)CD16? NK cell-like phenotype and correlates with HIV viral load.
| S-EPMC3447005 | biostudies-literature
Unknown Characterization of CD56-/CD16+ natural killer (NK) cells: a highly dysfunctional NK subset expanded in HIV-infected viremic individuals.
| S-EPMC549494 | biostudies-literature
Unknown Circulating CD56<sup>bright</sup> NK cells inversely correlate with survival of melanoma patients.
| S-EPMC6418246 | biostudies-literature
Unknown Activated Platelets Convert CD14<sup>+</sup>CD16<sup>-</sup> Into CD14<sup>+</sup>CD16<sup>+</sup> Monocytes With Enhanced FcγR-Mediated Phagocytosis and Skewed M2 Polarization.
| S-EPMC7817612 | biostudies-literature
Unknown Deficient IL-2 Produced by Activated CD56<sup>+</sup> T Cells Contributes to Impaired NK Cell-Mediated ADCC Function in Chronic HIV-1 Infection.
| S-EPMC6648879 | biostudies-literature
Unknown The aryl hydrocarbon receptor modulates the function of human CD56<sup>bright</sup> NK cells.
| S-EPMC6658132 | biostudies-literature
Unknown Single-cell profiling reveals periventricular CD56<sup>bright</sup> NK cell accumulation in multiple sclerosis.
| S-EPMC9135404 | biostudies-literature