Ontology highlight
ABSTRACT:
SUBMITTER: Sen K
PROVIDER: S-EPMC86632 | biostudies-literature | 2000 May
REPOSITORIES: biostudies-literature
Journal of clinical microbiology 20000501 5
Yersinia enterocolitica accounts for 50% of the clinical sepsis episodes caused by the transfusion of contaminated red blood cells. A 5' nuclease TaqMan PCR assay was developed to detect Y. enterocolitica in blood. Primers and a probe based on the nucleotide sequence of the 16S rRNA gene from Y. enterocolitica were designed. Whole-blood samples were spiked with various numbers of Y. enterocolitica cells, and total chromosomal DNA was extracted. When the TaqMan PCR assay was performed, as few as ...[more]