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Validated 5' nuclease PCR assay for rapid identification of the genus Brucella.

ABSTRACT: A real-time, genus-specific 5' nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.

SUBMITTER: Bogdanovich T 

PROVIDER: S-EPMC404622 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature

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Validated 5' nuclease PCR assay for rapid identification of the genus Brucella.

Bogdanovich T T   Skurnik M M   Lübeck P S PS   Ahrens P P   Hoorfar J J  

Journal of clinical microbiology 20040501 5

A real-time, genus-specific 5' nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs. ...[more]

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