Involvement of lncRNA IL21-AS1 in interleukin-2 and T follicular regulatory cell activation in systemic lupus erythematosus.

ABSTRACT:

Background

The single nucleotide polymorphism (SNP) rs62324212, located in IL21 antisense RNA 1 (IL21-AS1), has been identified as a genetic risk variant associated with systemic lupus erythematosus (SLE). We aimed to probe the characteristics of IL21-AS1 and explore its clinical relevance focusing on T helper subsets and disease activity in patients with SLE.

Methods

rs62324212 genotyping was determined using allelic discrimination by quantitative PCR. Gene expression in peripheral blood mononuclear cells and cell surface markers in CD4⁺ T cells were analyzed using PCR and flow cytometry. The association among IL21-AS1, CD4⁺ T cell subsets, and SLE disease activity was accessed.

Results

Ensembl Genome Browser analysis revealed that rs62324212 (C

SUBMITTER: Hao H 

PROVIDER: S-EPMC8665514 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature