<i>Rpl24^Bst</i> mutation suppresses colorectal cancer by promoting eEF2 phosphorylation via eEF2K.

ABSTRACT: Increased protein synthesis supports the rapid cell proliferation associated with cancer. The Rpl24^Bst mutant mouse reduces the expression of the ribosomal protein RPL24 and has been used to suppress translation and limit tumorigenesis in multiple mouse models of cancer. Here, we show that Rpl24^Bst also suppresses tumorigenesis and proliferation in a model of colorectal cancer (CRC) with two common patient mutations, Apc and Kras. In contrast to previous reports, Rpl24^Bst mutation has no effect on ribosomal subunit abundance but suppresses translation elongation through phosphorylation of eEF2, reducing protein synthesis by 40% in tumour cells. Ablating eEF2 phosphorylation in Rpl24^Bst mutant mice by inac

SUBMITTER: Knight JR 

PROVIDER: S-EPMC8668188 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature