Preclinical testing of oncolytic adenovirus sensitivity in patient-derived tumor organoids.
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ABSTRACT:
SUBMITTER: Pascual-Sabater S
PROVIDER: S-EPMC8672098 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Preclinical testing of oncolytic adenovirus sensitivity in patient-derived tumor organoids.
Pascual-Sabater Silvia S Raimondi Giulia G Mato-Berciano Ana A Vaquero Eva C EC Ausania Fabio F Fillat Cristina C
STAR protocols 20211211 4
Patient-derived organoids (PDOs) have shown the potential to reflect patient sensitivity to chemotherapeutic or targeted drugs. Recently, we showed that organoid models can also serve as a platform to screen for selectivity and potency of oncolytic adenoviruses (OAds). In this protocol, we describe the steps for tumor organoid adenoviral infection and functional assessment of patient-specific responses to OAds. We provide methods to determine OAd relative efficacy by evaluation of PDO viability ...[more]
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Project description:Interventions: case series:Nil
Primary outcome(s): pdo modeling success rate(%)
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