Ontology highlight
ABSTRACT:
SUBMITTER: Kimmel BR
PROVIDER: S-EPMC8672447 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20200303 10
This paper presents a solid-phase strategy to efficiently assemble multiprotein scaffolds-known as megamolecules-without the need for protecting groups and with precisely defined nanoscale architectures. The megamolecules are assembled through sequential reactions of linkers that present irreversible inhibitors for enzymes and fusion proteins containing the enzyme domains. Here, a fusion protein containing an N-terminal cutinase and a C-terminal SnapTag domain react with an ethyl <i>p</i>-nitrop ...[more]