A high-throughput multiplexing and selection strategy to complete bacterial genomes.

ABSTRACT:

Background

Bacterial whole-genome sequencing based on short-read technologies often results in a draft assembly formed by contiguous sequences. The introduction of long-read sequencing technologies permits those contiguous sequences to be unambiguously bridged into complete genomes. However, the elevated costs associated with long-read sequencing frequently limit the number of bacterial isolates that can be long-read sequenced. Here we evaluated the recently released 96 barcoding kit from Oxford Nanopore Technologies (ONT) to generate complete genomes on a high-throughput basis. In addition, we propose an isolate selection strategy that optimizes a representative selection of isolates for long-read sequencing considering as input large-scale bacterial collections.

Results

De

SUBMITTER: Arredondo-Alonso S 

PROVIDER: S-EPMC8673558 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature