Ontology highlight
ABSTRACT:
SUBMITTER: Karsi A
PROVIDER: S-EPMC2663204 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Karsi Attila A Gülsoy Nagihan N Corb Erin E Dumpala Pradeep R PR Lawrence Mark L ML
Applied and environmental microbiology 20090205 7
A high-throughput bioluminescence screening procedure for identification of virulence genes in bacteria was developed and applied to the fish pathogen Edwardsiella ictaluri. A random transposon mutant library expressing bioluminescence was constructed and robotically arrayed on 384-well plates. Mutants were cultivated and mixed with catfish serum and neutrophils in 96-well plates, and bioluminescence was used to detect mutants that are more susceptible to killing by these host factors. The virul ...[more]