Ontology highlight
ABSTRACT:
SUBMITTER: Stender EGP
PROVIDER: S-EPMC8674230 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Stender Emil G P EGP Ray Soumik S Norrild Rasmus K RK Larsen Jacob Aunstrup JA Petersen Daniel D Farzadfard Azad A Galvagnion Céline C Jensen Henrik H Buell Alexander K AK
Nature communications 20211215 1
Liquid-liquid phase separation or LLPS of proteins is a field of mounting importance and the value of quantitative kinetic and thermodynamic characterization of LLPS is increasingly recognized. We present a method, Capflex, which allows rapid and accurate quantification of key parameters for LLPS: Dilute phase concentration, relative droplet size distributions, and the kinetics of droplet formation and maturation into amyloid fibrils. The binding affinity between the polypeptide undergoing LLPS ...[more]