Unknown

Dataset Information

0

A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry.


ABSTRACT: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has created an urgent need for new technologies to treat COVID-19. Here we report a 2'-fluoro protected RNA aptamer that binds with high affinity to the receptor binding domain (RBD) of SARS-CoV-2 spike protein, thereby preventing its interaction with the host receptor ACE2. A trimerized version of the RNA aptamer matching the three RBDs in each spike complex enhances binding affinity down to the low picomolar range. Binding mode and specificity for the aptamer-spike interaction is supported by biolayer interferometry, single-molecule fluorescence microscopy, and flow-induced dispersion analysis in vitro. Cell culture experiments using virus-like particles and live SARS-CoV-2 show that the aptamer and, to a larger extent, the trimeric aptamer can efficiently block viral infection at low concentration. Finally, the aptamer maintains its high binding affinity to spike from other circulating SARS-CoV-2 strains, suggesting that it could find widespread use for the detection and treatment of SARS-CoV-2 and emerging variants.

SUBMITTER: Valero J 

PROVIDER: S-EPMC8685691 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC9234021 | biostudies-literature
| S-EPMC7888609 | biostudies-literature
| S-EPMC7310621 | biostudies-literature
| S-EPMC8789370 | biostudies-literature
| S-EPMC8290372 | biostudies-literature
| S-EPMC10302749 | biostudies-literature
| S-EPMC7720121 | biostudies-literature
| S-EPMC9868516 | biostudies-literature
| S-EPMC8310277 | biostudies-literature
| S-EPMC10669532 | biostudies-literature