Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release.
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ABSTRACT:
SUBMITTER: Kapoor T
PROVIDER: S-EPMC8689351 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Publications
Time-lapse imaging of <i>Drosophila</i> testis for monitoring actin dynamics and sperm release.
Kapoor Tushna T Dubey Pankaj P Ray Krishanu K
STAR protocols 20211215 1
Here we describe a simple step-by-step protocol for collecting high-resolution, time-lapse images of intact <i>Drosophila</i> testis <i>ex vivo</i> for a limited period using a confocal microscope, with minimum photo-toxic damage, to monitor spermatid individualization, coiling, and release. The F-actin dynamics during spermatid morphogenesis can be further investigated through laser ablations, Fluorescence-Recovery-After-Photobleaching, and drug treatments, using this protocol. For complete det ...[more]
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